human liver tissues frozen human liver sections  (Novus Biologicals)

Journal: bioRxiv

Article Title: Using a barcoded AAV capsid library to select for novel clinically relevant gene therapy vectors

doi: 10.1101/683672

Figure Lengend Snippet: (A) Representative immunofluorescence images from livers of mice injected with ssAAV-Td Tomato Red at 1E11 vg i.v. with varying capsid serotypes (3 mice for DJ, 4 mice each for LK03 and KP1). DAPI (blue), human-specific FAH (green), and Tomato Red (red) on liver sections. Scale bar, 50 µM. (B) Quantification of human hepatocyte repopulation levels, transduction efficiency for all hepatocytes as well as human hepatocytes. Each data point represents an area of interest for each mouse. A total of six to nine areas of interest for each mouse were scanned and analyzed. The mean and standard deviation for each mouse is indicated. Only statistically significant differences between the groups are shown in the graphs. **: p<0.01, ****: p<0.0001

Article Snippet: Antibody validity controls included secondary-only staining and demonstration on positive control human liver tissue sections (Cat. no. HF-314; Zyagen) and negative control untreated mouse liver sections (Cat. no. MF-314-C57; Zyagen).

Techniques: Immunofluorescence, Injection, Transduction, Standard Deviation